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  • Analyze Cells
    with Park Scanning Probe Microscopy

Brian Choi, Bio-application scientist
For more information, please contact app@parksystems.com
Data Reference: Gordon Jung, Application Scientist

Cell imaging in live status provides critical insight into the fundamental nature of cell and tissue function. Especially importantly, imaging a cell membrane in high resolution and monitoring its dynamics can reveal a variety of cellular processes and cell signaling behavior. To capture the real nature of cell membrane dynamics, it is important that the cell’s physiology is not interrupted by imaging technique in use. Scanning ion conductance microscopy (SICM) is a non-contact surface morphology imaging technique, operating in an ion-containing liquid environment. SICM provides automatic time-lapsed images and shows real-time changes in a cell membrane’s small features during imaging.

  • Monitor the dynamics of cell membrane and nanostructures of live cell
  • Automatic running mode (ARS) visualizes and records morphology changes of live cell membrane


cell-membrane-c2c12Cell Membrane of C2C12 Conditions:
Cell line: : C2C12, Myoblast, Scan size: 15 μm x 20 μm
Glass pipette, ARS mode, Medium: 1x PBS with 10% DMEM


In this study, the cell membrane morphology of living C2C12 (myoblast) and HeLa cells, as well as the dynamics of those membranes, were captured by SICM over time. The continuous imaging of cell membranes unveils the detail morphological physiology of C2C12 and HeLa cells by showing outward cellular extensions, such as microviili, covering the plasma membrane. It is clearly observed that the microvilli pops down crossing the cell membrane (C2C12), and those cellular extensions are transforming its structure and moving the position dynamically in the lateral direction as time passes (HeLa cell).


cell-membrane-live-helaCell Membrane of Live Hela Conditions:
Cell line: Hela cell, Scan size: 30 μm x 15 μm
Glass pipette, ARS mode, Medium: 1x PBS with 10% DMEM


Park Cell Analysis Systems

  park-analyze-cell-nx12-bio park-analyze-cell-nx10 park-analyze-cell-xe7
  Park NX12-Bio Park NX10 Park XE7
Scanning Ion Conductance Microscopy (SICM)  
Atomic Force Microscopy (AFM) with liquid probe hand
Inverted Optical Microscopy (IOM)    
Live Cell Chamber